Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process
2013
Geddes, C.C. | Mullinnix, M.T. | Nieves, I.U. | Hoffman, R.W. | Sagues, W.J. | York, S.W. | Shanmugam, K.T. | Erickson, J.E. | Vermerris, W.E. | Ingram, L.O.
A process was developed for seed culture expansion (3.6 million-fold) using 5% of the hemicellulose hydrolysate from dilute acid pretreatment as the sole organic nutrient and source of sugar. Hydrolysate used for seed growth was neutralized with ammonia and combined with 1.0mM sodium metabisulfite immediately before inoculation. This seed protocol was tested with phosphoric acid pretreated sugarcane and sweet sorghum bagasse using a simplified process with co-fermentation of fiber, pentoses, and hexoses in a single vessel (SScF). A 6h liquefaction (L) step improved mixing prior to inoculation. Fermentations (L+SScF process) were completed in 72h with high yields (>80gal/USton). Ethanol titers for this L+SScF process ranged from 24g/L to 32g/L, and were limited by the bagasse concentration (10% dry matter).
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