Isolation and characterization of a soybean hsp70 gene
1991
Roberts, J.K. | Key, J.L.
Soybean, like many other organisms, responds to an increase in growth temperature by producing a set of new proteins, heat shock proteins. The heat shock proteinshave been classified into several categories according to their molecular weight. Data are presented on theisolation, sequence characterization, and expression of a 70 kDa heat shock protein gene from soybean. AcDNA clone was isolated using a Drosophila hsp70 clone as a heterologous probe, and the cDNA wasused for isolation of the soybean gene corresponding to the cDNA. The structure of this soybean isvery similar to the hsp70 genes from other organisms. It has several sequences in the 5' untranscribedregion that are similar to the well characterized heat shock consensus element found in otherorganisms. These heat shock consensus elements have the expected position relative to the start of transcription. Unlike hsp70-like genes previously isolated from other plants, this gene does not have anintron. This protein shows high amino acid sequence similarity to other hsp70 proteins from suchdiverse organisms as Drosophila, rat, and Xenopus. This soybean gene is only expressed during heat shock.In addition to the hsp70 gene isolated here, there is evidence for many other hsp70-like genes insoybean.
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