Sustainable production of azadirachtin from differentiated in vitro cell lines of neem (Azadirachta indica)
2013
Singh, Mithilesh | Chaturvedi, Rakhi
Neem possesses immense medicinal properties and has immediate application as ecofriendly, biodegradable biopesticide. All these properties are because of a structurally complex bioactive compound Azadirachtin, mainly present in seeds. However, it could not be exploited to its full because of heterogeneity in compound production due to out-breeding in nature of the plant. This is the first elaborate report on systematic studies on azadirachtin biosynthesis where different redifferentiated and dedifferentiated in vitro cell lines of A. indica (neem), obtained from various explants, were utilized. All cell lines were found positive for the compound with significantly higher azadirachtin yield of 2330 µg /g DW was obtained from redifferentiated cell lines established from zygotic embryo cultures. The study, demonstrates the possibility of consistent biosynthesis of azadirachtin in bulk under optimal growth conditions.
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