In vitro plantlet regeneration from protocorms of Dendrobium lituiflorum Lindl. and Cymbidium bicolor Lindl. and their acclimatisation: effect of salts, sucrose, and banana extract
2012
The salts present in modified Knudson C (KC) medium (1946), 2% (w/v) sucrose, and a natural banana extract (BE) [1 – 20% (v/v)] were studied singly, or in combination, for their effects on the regeneration of protocorms of the orchid species, Dendrobium lituiflorum and Cymbidium bicolor. The incorporation of KC salts along with higher percentages of BE [10% or 20% (v/v)] promoted protocorm development as well as shoot and root formation. The presence of 2% (w/v) sucrose in the medium further enhanced root lengths and the rooting percentage, irrespective of the presence of KC salts. Incorporation of both KC salts and 2% (w/v) sucrose was found to be obligatory, in conjunction with BE (a cost-effective natural additive) for plantlet regeneration. In vitro-raised plantlets of both orchid species exhibited high rates of survival under greenhouse conditions. Scanning electron microscopy revealed elliptical stomata and epicuticular granular wax deposits on their abaxial leaf surfaces after acclimatisation.
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