Optimizing environmental factors for large-scale multiplication of chrysanthemum (chrysanthemum grandiflorum) in balloon-type bioreactor culture
2005
We have developed optimum culture conditions for the large-scale propagation of chrysanthemum in balloon-type bioreactors to achieve vigorous growth and quality. The effects of NH₄⁺⁺/NO₃⁻⁻ ratio, air volume, air temperature, photosynthetic photo flux, and an inoculation density on the growth and quality of plantlets were investigated. The best production conditions were an NH₄⁺⁺:NO₃⁻⁻ ratio of 20:40 mM, air exchange of 0.1 vvm min⁻⁻¹, air temperature 25°°C, photosynthetic photo flux (PPF) at 100 μμmol m⁻⁻² s⁻⁻¹, and an inoculation density of 40 nodes Chrysanthemum grandiflorum. Under each of these conditions, the maximum growth rate reached 279.0, 260.0, 20.0, 23.3, and 94.5 (g-fresh weight per plantlet d⁻⁻¹), respectively, at 12 wk of culture. These results specify the key environmental factors that can be regulated to improve the quality and quantity of flowers and increase yield in large-scale bioreactor cultures of chrysanthemum.
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