Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity
2020
In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated. Meanwhile, the mechanism of β-glucan biosynthesis was investigated by RNA-Seq. Based on the results, C. militaris growing at an initial growth pH of 5–7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8–9 (CMsB) and their unigenes showed the comparable expression. The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA. The results of RNA-seq showed 1088 differentially expressed genes between CMsA and CMsB groups. Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups. In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.
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