Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3
2022
Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO₃ to the shoot regeneration medium (MS medium supplemented with 30 g L⁻¹ sucrose, 100 mg L⁻¹ myoinositol, and 2.25 BA mg L⁻¹). Five concentrations of AgNO₃ (2, 4, 6, 8, and 10 mg L⁻¹) were used in addition to a control. The effect of AgNO₃ on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L⁻¹. Enhancements in shoot regeneration were attributed to the known role of AgNO₃ as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H₂O₂ content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO₃ concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H₂O₂ content, lipid peroxidation, and DNA damage.
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