Enhancing 6-APA Productivity and Operational Stability of Penicillin G Acylase via Rapid Surface Capping on Commercial Resins
2016
Yang, Dong | Liu, Hua | Shi, Jiafu | Wang, Xueyan | Zhang, Shaohua | Zou, Hongjian | Jiang, Zhongyi
In this study, immobilized penicillin G acylase (PGA) was prepared via a facile and rapid approach of generating the TA-Tiᴵⱽ layer on PGA-adsorbed commercial resins (PGA@Resins). In brief, the TA-Tiᴵⱽ layer was constructed through coordination-enabled self-assembly of tannic acid (TA) and titanium(IV) bis(ammonium lactate) dihydroxide (Ti-BALDH). In comparison to PGA@Resins, TA-Tiᴵⱽ-capped PGA@Resins exhibited higher 6-aminopenicillanic acid (6-APA) productivity and enhanced operational stability along with comparable activity recovery during the catalytic hydrolysis of penicillin G potassium (PGK). Particularly, TA-Tiᴵⱽ-capped PGA@Resins exhibited relative activities of 103.7% and 81.51%, respectively, after 68-day storage and 20 cycles, indicating significantly enhanced storage and recycling stabilities compared to PGA@Resins (68.98% and 62.88%). Both immobilized PGA were further packed into a glass column for hydrolyzing PGK in a continuous flow reactor, where TA-Tiᴵⱽ-capped PGA@Resins displayed a much higher 6-APA yield (initial yield: 49.22% vs 28.99%; yield after 10 days: 17.39% vs 6.11%) than PGA@Resins.
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