Chemical structure of preinhibitin in citrus against citrus stem-end rot
1992
Homma, Y. | Ohsawa, T. | Arimoto, Y. | Takahashi, H.
When pedicels of Satsuma mandarin fruits on trees were inoculated with Diaporthe citri and then harvested in early December and stored at room temperature, stem-end rot was not observed until late February. Numerous crystals were distributed in the tissues around the stem button. The dissolved crystals inhibited conidial germination and germ-tube elongation of D. citri. The substance, referred to as the preinhibitin, was hydrolyzed by refluxing in ethyl alcohol-hydrochloric acid, 10:1, for 18 h. The resinous material obtained from the ether-soluble fraction was purified by repeated preparative thin-layer chromatography on silica gel to yield pale yellow crystals. This compound was identified as a flavanone, hesperetin. The original compound proved to be a glycoside with hesperetin as the aglycon. The preinhibitin was identified as hesperetin-7-rhamnoglucoside or hesperidin.
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