Coleosporium plumeriae, causal agent of plumeria rust fungi in Yogyakarta, Indonesia, showed DNA sequence variation
2022
Widiastuti, Ani | Santika, Islaminati Anna
Plumeria rust fungi caused by Coleosporium plumeriae is one of emerging diseases which is currently fast distributed over the world. The pathogen is also listed as an invasive plant pathogen species. This research aimed to identify Plumeria Rust Fungi from five regencies in Yogyakarta, Indonesia based on Rust2inv and LR6 Primers, which was developed from 5.8S subunit, ITS2 and 28S LSU regions to get more accurate identification of C. plumeriae. The results showed that uredospores of each specimen had variation in shape and verrucose spore walls thickness. All five specimens, which were collected from Plumeria rubra and P. alba, were identified as C. plumeriae based on homology sequences from BLAST NCBI and phylogenetic tree. Visualisation of nucleotides sequence alignment confirmed that those five specimens contain some nucleotides variations. Especially, BL specimen showed nucleotides insertions and substitutions, different from other sequences. The result performed a basic significant information for further study about genetic variations and pathogen evolution of C. plumeriae. Each specimen sequence in this study was deposited into the GenBank with accession No. OM780189- OM78019.
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