Calcitonin receptor bindings in the hen hypothalamus before and after oviposition
2011
Nakayama, H. | Takahashi, T. | Funaki, W. | Nakagawa-Mizuyachi, K. | Kawashima, M.
To demonstrate the presence of a receptor for calcitonin (CT) in the hen hypothalamus and to determine when CT acts on this tissue during the oviposition cycle, bindings of ¹²⁵I labeled CT in the plasma membrane fraction of the hen hypothalamus were measured by radioligand binding assay. The specific CT binding component in the plasma membrane fraction of the hypothalamus containing the preoptic area (HPOA) possessed properties of a receptor: binding specificity to CT, saturable binding, high affinity, and limited capacity. As for the median eminence area, no specific binding component was found in the present study. Therefore, the binding component for CT in the plasma membrane fraction of HPOA is likely to be a receptor for CT. In laying hens, the binding affinity of CT receptor increased at 30 min before oviposition and the binding capacity was decreased at 30 min before oviposition but not changed in nonlaying hens during a 24-h period. These results suggest that the action of CT on the hen HPOA may increase 30 min before oviposition.
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