Regulation of ammonium ion assimilation enzymes in Neurospora crassa nit-2 and ms-5 mutant strains
1993
Calderon, J. | Martinez, L.M.
In Neurospora crassa the nit-2 and nmr-1 (ms-5) loci represent the major control genes encoding regulatory proteins that allow the coordinated expression of various systems involved with the utilization of a secondary nitrogen source. In this paper we examine the effect of the nit-2 and ms-5 (nmr-1 locus) mutations on the regulation of the ammonium assimilation enzymes, glutamine synthetase and glutamate dehydrogenase, which are regulated by the products of these genes; however, glutamate synthase is not so regulated. Glutamine synthetase and glutamate dehydrogenase levels are also regulated by the amino nitrogen content. We present evidence that the ms-5 and gln(r) strains, which behave very similarly in their resistance to glutamine repression, are different and map in different loci.
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