Immunobiosensor determination of beta-agonists in urine using integrated immunofiltration clean-up
2004
Johansson, M.A. | Hellenas, K.E.
A fast immunobiosensor assay for screening of β-agonists in urine, using integrated immunofiltration clean-up with 30 kDa cutoff centrifugal filter, was developed. The same antibody aliquot was used for clean-up and biosensor analysis, and no organic solvent was needed. Matrix interference from urine was efficiently reduced. Two antibodies with different cross-reactivities were compared. Although both antibodies were able to detect clenbuterol (CBL) below 1 ng mL-1, a polyclonal antibody directed against salbutamol was chosen because of its ability to recover several β-agonists. The decision limit (CCα) of the final assay was 0.29 ng mL-1, and an α-error of 1% gave a β-error of <1% for twenty reference bovine samples spiked with 1 ng mL-1 CBL.
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