Unusual partially 3-O-methylated α-galactan from mushrooms of the genus Pleurotus
2008
Carbonero, E.R. | Gracher, A.H.P. | Rosa, M.C.C. | Torri, G. | Sassaki, G.L. | Gorin, P.A.J. | Iacomini, M.
Indistinguishable partially 3-O-methylated galactans were isolated from the edible basidiomycetes Pleurotus eryngii and Pleurotus ostreatoroseus. They were obtained via successive aqueous extraction, freeze-thawing, precipitation with Fehling solution of soluble material, and ultrafiltration. Mono- and bidimensional 13C and 1H-nuclear magnetic resonance spectroscopy (HMBC, HETEROTOCSY, COSY, and HMQC), and methylation analysis were used to determine their structures. They were linear, partially 3-O-methylated, (1 lead to 6)-linked α-d-galactans containing Gal and 3-Me-Gal, in a 3:1 molar ratio (GC-MS of alditol acetates).
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