A simple, room-temperature extraction method for 14carbon-labeled red raspberry leaf tissues
1993
Deng, R. | Donnelly, D.
Labeled ((14)C) Compounds were recovered from tissue disks taken from (14)CO2-fed leaves of 1-year-old greenhouse-grown plants and 1-month-old ex vitro transplants of red raspberry (Rubus idaeus L.) by hot (boiling in 80% ethanol immediately after (14)C exposure), delayed-hot (boiling in 80% ethanol after a 2- to 3-day ethanol soak), and room- temperature (RT) (2- to 3-day soak in 80% ethanol) extraction methods. The RT extraction method was simple but as effective for extracting (14)C-labeled compounds from red raspberry leaf tissues as hot and delayed-hot extraction methods.
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