Hydrogen peroxide generation in organelles of Euglena gracilis
1993
Ishikawa, T. | Takeda, T. | Shigeoka, S. | Hirayama, O. | Mitsunaga, T.
Hydrogen peroxide (H2O2) generation in intact mitochondria and chloroplasts was investigated in Euglena gracilis. In mitochondria, the addition of respiratory substrate caused the formation of Lactate, the most effective substrate, yielded at the rate of 9.5 nmol min-1 mg protein-1. Chloroplasts showed a rate of generation of 5 micromole mg chlorophyll-1 hr-1 under illumination. The results reported here demonstrate that generated in mitochondria and chloroplasts and immediately diffuses from each organelle into the cytosol.
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