Enantiomeric composition of monoterpene hydrocarbons in some conifers and receptor neuron discrimination of alpha-pinene and limonene enantiomers in the pine weevil, Hylobius abietis
1998
Wibe, A. | Borg-Karlson, A.K. | Persson, M. | Norin, T. | Mustaparta, H.
The enantiomeric composition of seven monoterpene hydrocarbons in headspace volatiles of spruce sawdust and seedlings (Picea abies), pine seedlings (Pinus sylvestris), and branches of juniper (Juniperus communis) was determined by gas chromatographic separation on a beta-Cyclodextrin column. For the six monoterpenes, alpha-pinene, camphene, beta-pinene, sabinene, limonene, and beta-phellandrene, both enantiomers were present, whereas for 3-carene only the (+)-configuration was found. The amount of each enantiomer varied considerably both in relation to total amount of all of them, and for the six pairs also in relation to the opposite enantiomer. One olfactory receptor neuron in the pine weevil (Hylobius abietis) showed a strong response to alpha-pinene when stimulated with all four headspace materials via a GC equipped with a DB-WAX column. The same neuron was subsequently tested with repeated stimulations via the GC effluent containing the (+)- or (-)- enantiomer. A marked better response to (+)- than to (-)-alpha-pinene was elicited. Another olfactory receptor neuron that responded strongly to limonene when stimulated with the spruce volatiles was tested for enantiomers of limonence. This neuron responded more strongly to (-)- than to (+)-limonene, when stimulated alternately with each of the limonene enantiomers. Discrimination between enantiomers by plant olfactory receptor neurons suggests that the enantiomeric ratios of volatile compounds may be important in host location by the pine weevil.
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