Effects of histone hyperacetylation on the preimplantation development of male and female bovine embryos
2010
Oliveira, Clara S. | Saraiva, Naiara Z. | de Souza, Marcela M. | Tetzner, Tatiane A.D. | de Lima, Marina R. | Garcia, Joaquim M.
Trichostatin A (TSA) induces histone hyperacetylation by inhibiting histone deacetylases and consequently increasing gene expression. The hypothesis was that TSA supplementation during the in vitro culture (IVC) of bovine embryos would increase the blastocyst rate, particularly in low-quality and female embryos. Oocytes were fertilised separately with X and Y spermatozoa and, 70h after IVF, the IVC medium was supplemented with 5nM and 15nM TSA for 48 or 144h. Incubation of female embryos with 5nM and 15nM TSA resulted in similar increases in acetylated histone H3K9 levels. However, to see comparable effects on acetylated histone H3K9 levels in male embryos, the culture medium needed to be supplemented with 15nM TSA (as opposed to 5nM TSA for female embryos). Treatment of male and female embryos with 5nM TSA for 48h or female embryos with 5nM for 144h had no effect on blastocyst rates, although 15nM TSA compromised embryonic development. The terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay revealed increased apoptosis in female embryos treated with 5nM TSA for 144h, as well as in male and female embryos treated with 15nM TSA for 48h, but this increase in apoptosis was not observed in low-quality embryos. The results of the present study suggest that TSA treatment promotes histone hyperacetylation, but has no beneficial effects on the in vitro production of male and female bovine embryos during preimplantation development.
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