Mode of cultivation is critical for the optimal expression of recombinant hirudin by Saccharomyces cerevisiae
1993
Ibba, M. | Bonarius, D. | Kuhla, J. | Smith, A. | Kuenzi, M.
A repeated fed-batch process for the production of recombinant hirudin by S. cerevisiae was established. The efficiency and stability of protein synthesis was investigated using various media. High cell densities were attained with both synthetic and complex media and all cultures were stable throughout the investigation (30 days). Repeated fed-batch cultivation gave consistently higher hirudin yields than continuous cultivation on all media tested, with complex medium giving the highest yields. This was shown not to be a result of plasmid copy number differences but transcriptional control, despite the use of the constitutive GAPFL promoter.
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