Chemical composition and anti-hepatoma effect of Annona squamosa L. pericarp oil
2021
Chen, Ya-Yun | Li, Fu-Qiang | Zhu, Xiao-Li | Chen, Jian-Wei | Li, Xiang
In this study, an optimal method used to extract Annona squamosa pericarp oil (ASPO) was established according to the response surface model. The yield of ASPO was 1.45%. 8 fatty acids were identified from ASPO by GC-MS. Among them, (9Z)-9-Octadecenoic acid was abundant and accounted for 49.65%. The anti-hepatoma activities of ASPO were investigated against SMMC-7721 cell line in vitro and H₂₂ cell line in vivo. Proteins associated with apoptosis in tumour tissue were quantified by western blot assay. The result revealed that ASPO had significant anti-hepatoma activities with IC₅₀ value of 15.96 μg/mL in vitro and tumour inhibition rate of 54.14% at 50 mg/kg dose in vivo. Protein analysis showed that ASPO activated apoptosis by down-regulating Bcl-2, up-regulating Bax, cleaving caspase 9, cleaving caspase 8 and cleaving caspase 3 proteins. The possible mechanisms of apoptosis induced by ASPO were related to Fas/FasL/caspase-8/caspase-3 and Bcl-2/bax/caspase-9/caspase-3 pathways.
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