The purification and characterisation of allergenic hazelnut seed proteins
2008
Rigby, Neil M. | Marsh, Justin | Sancho, Ana I. | Wellner, Klaus | Akkerdaas, Jaap | Ree, Ronald van | Knulst, Andre | Fernández-Rivas, Montserrat | Brettlova, Vlasta | Schilte, Piet P. | Summer, Colin | Pumphrey, Richard | Shewry, P. R. (Peter R) | Mills, E.N Clare
A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.
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