Graphene-Orange II composite nanosheets with electroactive functions as label-free aptasensing platform for “signal-on” detection of protein
2013
Guo, Yujing | Han, Yujie | Guo, Yanxiu | Dong, Chuan
The combination of novel nanomaterials and biomolecule recognition units is promising in developing sensitive biosensors. This paper presents a facile approach for the preparation of water-soluble electroactive dye-Orange II functionalized graphene nanosheets (O-GNs). The successful attachment of Orange II on the surface of graphene nanosheets not only prevents the agglomeration of the as-formed graphene nanocomposite in aqueous media, but also endows graphene nanosheets with excellent electroactive property. Integrating the unique properties of the as-prepared O-GNs (high conductivity, high specific surface area and electroactive function) with high affinity and specificity of aptamer, a new label-free electrochemical biosensing concept is demonstrated for the “signal-on” detection of targets such as thrombin and lysozyme. The present O-GNs-based aptasensor exhibits good current response to the above proteins. The linear ranges for thrombin and lysozyme are 1.0×10−12–4.0×10−10M and 5.0×10−12–7.0×10−10M, with the detection limit of 3.5×10−13M and 1.0×10−12M, respectively. In contrast to the common laborious and expensive labeling approaches, the present O-GNs based aptasensing process is highly simple, cost-efficient and does not need labeling and modification of aptamers or introduce additional electrochemical probes.
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