Analysis of Cyclic Peptides in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS
2007
Shen, Yan | Han, Chao | Chen, Junhui | Wang, Xiaoru
A new method for the analysis of cyclic peptides (Pseudostellarins) in traditional Chinese medicine, Pseudostellaria heterophylla (Miq.) Pax, was established by high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). The real samples were separated by a reversed-phase C₁₈ column using a binary eluent under gradient conditions. Six cyclic peptides were isolated and detected with a DAD detector. The Pseudostellarins components were identified by APCI-MS in a negative mode. In the negative ion APCI mode, the best sensitivity and the lowest detection limit for Pseudostellarins were obtained by using a mobile phase consisting of acetonitrile and pure water. The APCI spectra were characterized by [M-H]- peak. The parent ions [M-H]- with highest intensity was used for quantification of one cyclic peptide-Pseudostellarin B.
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