Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species
1999
Cespedes, A. | Garcia, T. | Carrera, E. | Gonzalez, I. | Martínez Fernández, Ángel | Hernandez, P.E. | Martin, R.
A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
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