New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry
2011
Ziganshin, Rustam | Arapidi, Georgii | Azarkin, Igor | Zaryadieva, Elena | Alexeev, Dmitry | Govorun, Vadim | Ivanov, Vadim
This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI–TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC–MS/MS using an Agilent 6520 Accurate-Mass Q-TOF.
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