Binding of mycoplasmas to solid phase adsorbents
2005
Szathmáry, Susan | Rajapakse, Nandani | Székely, Ibolya | Pitlik, E. | Bíró, Judit | Erdei, Noémi | Stipkovits, L.
The capture of mycoplasmas <i>(M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum </i>and<i> M. arthritidis)</i> based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.
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