DEGRADATION OF NON‐OPSONIZED 32P‐LABELLED E. COLI BY UNSTIMULATED MOUSE PERITONEAL MACROPHAGES: Evaluation of a Method
1980
ROLLAG, HALVOR
Five hours after the ingestion of non‐opsonized E. coli by mouse peritoneal macrophages in vitro. 60–70 per cent of the bacteria were killed, estimated as decrease in colony forming units. When employing ³²P‐labelled E. coli, 50–60 per cent of the radioactivity was released to the medium by the macrophages during the same period of time. The mean number of visually‐counted bacteria per macrophage was constant in the post‐ingestive period. The macrophages killed the bacteria without early destruction. The release of radioactivity seemed to reflect the degradation of bacteria in the macrophages.
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