NMR studies of acetan and the related bacterial polysaccharide, CR1/4, produced by a mutant strain of Acetobacter xylinum
1995
Colquhoun, I.J. | Defernez, M. | Morris, V.J.
Acetan is a bacterial polysaccharide produced by Acetobacter xylinum NRRL B42. Chemical mutagenesis of A. xylinum allowed selection of a mutant strain which produced a new polysaccharide. CR1/4. 2D NMR methods have been used to assign the 1H and 13C spectra of the two polvsaccharides and to determine that CR1/4 has the structure shown below. The total number of O-acetyl groups is slightly less than two per repeating unit, (...). The pentasaccharide side chain of acetan is truncated to a disaccharide unit in CR1/4, but the structures are otherwise identical. In particular, the degree of acetylation is about the same and the O-acetyl groups are located at the same positions in both polysaccharides.
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