Formation of phenylethanoid glycosides by Cistanche deserticola callus grown on solid media
2003
Ouyang, J. | Wang, X.D. | Zhao, B. | Wang, Y.C.
The optimal growth of Cistanche deserticola callus and formation of phenylethanoid glycosides (PeG) was at 25 degrees C with light irradiation intensity of 24 umol m-2 s-1 on solidified B5 media supplemented with 0.5 mg 6-benzylaminopurine l-1, 10 mg gibberellin l-1, 800 mg casein hydrolysate l-1 and 20 g sucrose l-1. After 30 d culture, the biomass reached 15.5 g dry wt callus l-1 medium and its PEG content was 10.7% (w/w). The PeG content was 42%-127% higher than those in explants.
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