Complete genome analysis of African swine fever virus isolated from domestic pigs during the first ASF outbreaks in India
2022
Senthilkumar, Dhanapal | Rajukumar, Katherukamem | Venkatesh, Govindarajulu | Singh, Fateh | Tosh, Chakradhar | Kombiah, Subbiah | Dubey, Chandan K. | Chakravarty, Amitav | Barman, Nagendra N. | Singh, Vijendra Pal
African swine fever (ASF), considered as the most dreadful swine disease due to its very high mortality, emerged in India in 2020. The complete genome analysis of ASF viruses isolated during the first outbreaks in India showed a few unique non‐synonymous mutations in MGF 369‐11L, MGF 505‐4R, K205R and B263R genes. Frame shifts in the protein coding sequences were observed in DP60R, ASFV‐G_ACD 00190, MGF 110‐10‐L‐MGF110‐14L fusion, MGF 360‐14L and I267L genes of Indian ASF viruses as compared to ASFV/Georgia/2007. Complete genome based phylogenetic analysis of p72‐genotype‐II viruses showed the clustering of Indian isolates with ASFV/Wuhan/2019 in a separate clade. Phylogenetic analysis of concatenated sequences of 14 open reading frames (ORF) having single nucleotide polymorphisms (SNP) showed distinct grouping of Indian ASFVs with other Asian ASFVs. This is the first complete genome characterization of ASF viruses isolated from domestic pigs in India. The results indicate that number of Tandem Repeat Sequence (TRS) in the intergenic region between I73R and I329L genes, and the 14 ORFs with SNP reported in this study could be the genetic determinants to differentiate the closely related p72‐genotype II viruses circulating in Asia.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Agricultural Library