Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains
2018
Parmaki, Stella | Vyrides, Ioannis | Vasquez, Marlen I. | Hartman, Viki | Zacharia, Irene | Hadjiadamou, Ioanna | Barbeitos, Catarina B.M. | Ferreira, Frederico C. | Afonso, Carlos A.M. | Drouza, Chrysoulla | Koutinas, Michalis
This work explores the potential for development of a lupanine valorization process evaluating different isolated microorganisms for their capacity to metabolize the alkaloid. Ecotoxicological assessment demonstrated that lupanine is toxic for Vibrio fischeri and Daphnia magna exhibiting EC₅₀ values of 89 mg L⁻¹ and 47 mg L⁻¹ respectively, while acting both as growth inhibitor for a monocotyledonous and as promoter for a dicotyledonous plant. Among the eight aerobic and anaerobic strains isolated and identified Rhodococcus rhodochrous LPK211 achieved 81% removal for 1.5 g L⁻¹ lupanine, while no end-products were detected by NMR constituting a promising microorganism for lupanine biodegradation. Moreover, Rhodococcus ruber LPK111 and Rhodococcus sp. LPK311 exhibited 66% and 71% of removal respectively, including potential formation of lupanine N-oxide. Pseudomonas putida LPK411 reached 80% of lupanine removal and generated three fermentation products potentially comprising 17-oxolupanine and lupanine derivatives with open ring structures enabling the development of alkaloid valorization processes.
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