Essential oil composition of sachalinmint from Norway detected by solid-phase microextraction and gas chromatography-mass spectrometry analysis
2002
Rohloff, J.
The essential oil of leaves and flowers of sachalinmint [Mentha sachalinensis (Briq.) Kudo] grown in Norway (Trondheim) has been studied by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry analysis (GC-MS). The essential oil content increased linearly in acropetal direction from 1.08% (0-20 cm plant height) to 1.75% (60-80 cm; young leaves and flowers). The steam-distilled samples showed a minor complex matrix with a very high menthol and a much lower menthone content (87.89 and 4.05%, respectively). From testing of HS-SPME unequilibrated exposure times ranging from 10 s to 5 min, an extraction time of 30 s was found to be sufficient to detect both low- and high-eluting compounds. Comparison of HS-SPME and steam-distilled samples established that the same tendencies of increasing menthol/menthone content in the basipetal/acropetal direction could be detected by both analysis methods. With regard to the extraction efficiency, HS-SPME gave additional detailed information about less important terpenic compounds.
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