Enzyme immunoassay for fumonisin B1 applied to corn-based food
1994
Usleber, E. | Straka, M. | Terplan, G.
Polyclonal antibodies against fumonisin B1 (FB1) were produced in rabbits after immunization with a FB1-keyhole limpet hemocyanin conjugate. Using these antibodies and a FB1-horseradish peroxidase conjugate in competitive direct enzyme immunoassay (EIA), the average detection limit for FB1 (buffer solutions) was 0.17ng/mL. The relative cross-reactions with FB1, fumonisin B2, and fumonisin B3 were 100%, 24%, and 55%, respectively. Corn samples were extracted with methanol/water and the diluted raw extracts directly analyzed by EIA. The raw extracts were purified by employing strong ion-exchange (SAX) cartridges and reanalyzed by both EIA and a HPLC method. The recoveries for FB1, from artificially contaminated corn at levels of 50 and 500 ng/g were 59.7% and 73.2% after EIA analysis of the raw extracts; the corresponding values for SAX extracts were 53.5% and 54.4% (EIA) and 62.3% and 65.6% (HPLC), respectively. Food samples (n = 19) from German retail stores (some were import products from Italy) were analyzed: 16 were found to contain FBI in a range from 12 to 1300 ng/g.
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