Amniculibacterium aquaticum gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a stream
2019
Chen, Wen-Ming | Guo, Ya-Ping | Sheu, Ceshing | Sheu, Shih-Yi
Strain KYPW7ᵀ, isolated from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of strain KYPW7ᵀ were Gram-stain-negative, aerobic, non-spore forming, non-motile rods and formed white colonies. Growth occurred at 15–30 °C (optimum 25 °C), at pH 6–8 (optimum pH 6.5) and with 0–1% NaCl (optimum 0%). Phylogenetic analyses based on 16S rRNA and coding sequences of 92 protein clusters showed that strain KYPW7ᵀ represents a novel genus in the family Flavobacteriaceae. The 16S rRNA gene sequence of strain KYPW7ᵀ was related to the species of the genera Chryseobacterium (91.8–96.0% sequence similarity), Bergeyella (95.1–95.8%), Cloacibacterium (94.5–95.7%), Daejeonia (95.6%) and Riemerella (94.0–95.0%). Strain KYPW7ᵀ showed less than 72% average nucleotide identity and less than 24% digital DNA–DNA hybridization identity compared to the type strains of related genera within the family Flavobacteriaceae. The predominant fatty acids were iso-C₁₅:₀ and iso-C₁₇:₀ 3-OH. The major isoprenoid quinone was MK-6 and the DNA G + C content was 36.8 mol%. The polar lipids had phosphatidylethanolamine, three uncharacterized aminophospholipids and an uncharacterized phospholipid. The polyamines contained homospermidine, putrescine and spermidine. On the basis of the genotypic and phenotypic data, strain KYPW7ᵀ represents a novel species of a new genus in the family Flavobacteriaceae, for which the name Amniculibacterium aquaticum gen. nov., sp. nov. is proposed. The type strain is KYPW7ᵀ (= BCRC 81123ᵀ = LMG 30598ᵀ = KCTC 62512ᵀ).
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