Effect of germination, under different environmental conditions, on saponins, phytic acid and tannins in lentils (Lens culinaris)
1997
Ayet, G. | Burbano, C. | Cuadrado, C. | Pedrosa, M.M. | Robredo, L.M. | Muzquiz, M. | Cuadra, C. de la | Castano, A. | Osagie, A.
The effect of germination conditions on some antinutrients of Lens culinaris var Magda 20 seeds were studied. The seeds were germinated at 20 degrees C under variable conditions of time, water and light. Quantitative analyses of the soyasapogenols, inositol phosphates and tannins were carried out by capillary gas chromatography, high-performance liquid chromatography and spectrophotometric techniques respectively. Germinated seeds at day 6 contained higher levels soyasapogenol B than the controls, whereas in general the tannin content was reduced. Total phytic acid amounts did not decrease after 3 days of germination but was greatly reduced after 6 days. This work shows that the optimal conditions to reduce some antinutritional factors (tannins and phytic acid) in lentils were 6 days of seed germination in dark and with alternate watering. Therefore, germination conditions offer a good opportunity to improve the nutritional quality of lentils.
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