Two streptokinase genes are expressed with different solubility in Escherichia coli W3110
1999
Pupo, Elder | Baghbaderani, Behnam A. | Lugo, Victoria | Fernández, Julio | Páez, Rolando | Torréns, Isis
The streptokinase (SK) gene from S. equisimilis H46A (ATCC 12449) was cloned in E. coli W3110 under the control of the tryptophan promoter. The recombinant SK, which represented 15% of total cell protein content, was found in the soluble fraction of disrupted cells. The solubility of this SK notably differed from that of the product of the SK gene from S. equisimilis (ATCC 9542) which had been cloned in E. coli W3110 by using similar expression vector and cell growth conditions, and occurred in the form of inclusion bodies.
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