Hydrophobins as aqueous lubricant additive for a soft sliding contact
2015
Lee, Seung-Hwan | Røn, Troels | Pakkanen, Kirsi I. | Linder, Markus
Two type II fungal hydrophobins, HFBI and FpHYD5, have been studied as aqueous lubricant additive at a nonpolar, compliant sliding contact (self-mated poly(dimethylsiloxane) (PDMS) contact) at two different concentrations, 0.1mg/mL and 1.0mg/mL. The two hydrophobins are featured as non-glycosylated (HFBI, m.w. ca. 7kDa) vs glycosylated (FpHYD5, m.w. ca. 10kDa) proteins. Far UV CD spectra of the two hydrophobins were very similar, suggesting overall structural similarity, but showed a noticeable difference according to the concentration. This is proposed to be related to the formation of multimers at 1.0mg/mL. Despite 10-fold difference in the bulk concentration, the adsorbed masses of the hydrophobins onto PDMS surface obtained from the two solutions (0.1 and 1.0mg/mL) were nearly identical, suggesting that a monolayer of the hydrophobins are formed from 0.1mg/mL solution. PDMS–PDMS sliding interface was effectively lubricated by the hydrophobin solutions, and showed a reduction in the coefficient of friction by as much as ca. two orders of magnitude. Higher concentration solution (1.0mg/mL) provided a superior lubrication, particularly in low-speed regime, where boundary lubrication characteristic is dominant via ‘self-healing’ mechanism. FpHYD5 revealed a better lubrication than HFBI presumably due to the presence of glycans and improved hydration of the sliding interface. Two type II hydrophobins function more favorably compared to a synthetic amphiphilic copolymer, PEO–PPO–PEO, with a similar molecular weight. This is ascribed to higher amount of adsorption of the hydrophobins to hydrophobic surfaces from aqueous solution.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Agricultural Library