Molecular and supra-molecular structure of waxy starches developed from cassava (Manihot esculenta Crantz)
2013
Rolland-Sabaté, Agnès | Sánchez, Teresa | Buléon, Alain | Colonna, Paul | Ceballos, Hernan | Zhao, Shan-Shan | Zhang, Peng | Dufour, Dominique
The aim of this work was to characterize the amylopectin of low amylose content cassava starches obtained from transgenesis comparatively with a natural waxy cassava starch (WXN) discovered recently in CIAT (International Center for Tropical Agriculture). Macromolecular features, starch granule morphology, crystallinity and thermal properties of these starches were determined. M¯w of amylopectin from the transgenic varieties are lower than WXN. Branched and debranched chain distributions analyses revealed slight differences in the branching degree and structure of these amylopectins, principally on DP 6–9 and DP>37. For the first time, a deep structural characterization of a series of transgenic lines of waxy cassava was carried out and the link between structural features and the mutated gene expression approached. The transgenesis allows to silenced partially or totally the GBSSI, without changing deeply the starch granule ultrastructure and allows to produce clones with similar amylopectin as parental cassava clone.
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