Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L
1994
Opsahl-Ferstad, H.G. | Bjornstad, A. | Roguli, O.A.
Genotypes of Lolium perenne L. with different androgenetic responses were used to test effects of induction medium composition. The media tested were potato II (pH), 190-2, and modified Linsmaier and Skoog media, LS-1, LS-2, and LS-3. The effect of different gelling agents, activated charcoal in a 'double layer' design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,959 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equivalent in production of green plants. Cold pretreatment of the anthers (4 degrees C) significantly increased the number of embryo-like structures, the number and proportion of albino plants produced, but not the production of green plants.
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