Rapid detection and identification of Erwinia carotovora subsp. atroseptica by a conjugated Staphylococcus aureus slide agglutination test
1992
McLeod, A. | Perombelon, M.C.C.
A conjugated Staphylococcus aureus slide agglutination test was used to detect and identify the potato blackleg pathogen, Erwinia carotovora subsp. atroseptica. Agglutination was obtained with > 10(8) cfu/ml of the homologous strain with a polyclonal antiserum (171) against E.c. atroseptica serogroup I which is the predominant E.c. atroseptica serogroup on potatoes in Scotland. The titre of antiserum 171 against live cells of E.c. atroseptica groups I and XXII was 2000 whereas that of other serogroups was considerably less; only 1 and 4 out of 22 serogroups of E. carotovora subsp. carotovora reacted at 1 : 1500 and 1 : 1000 antiserum dilutions, respectively and one of the three less common other E.c. atroseptica serogroups reacted at 1 : 1000. When tested against 24 different bacterial species including E. chrysanthemi and saprophytic bacteria present in potato tuber rots, negative results were obtained with 1 : 1000 antiserum dilution. The titre against heat-treated (1 h, 70 degrees C) cells of E.c. atroseptica serogroups I and XXII was 1700-2000 whereas it was < 10 against other bacteria including E.c. carotovora. Detection of E.c. atroseptica serogroups I and XXII in diseased potato tissues was achieved directly by the slide agglutination test, but lower antiserum dilutions (1 : 700-1000) were needed. Still lower antiserum dilutions were needed with heat-treated test material for E.c. atroseptica identification.
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