A method for evaluating the toxicity interaction of binary mixtures
2020
Wei, Qiu-yang
Improving the formulation of biological insecticides for greater efficiency and competitiveness is of particular importance with respect to the successful application of these agents in the field. In this regard, mixing different agents is known to be an effective strategy for enhancing practical pest control. However, traditional chemical-based control strategies have significant limitations that compromise control effectiveness, as effects tend to become increasingly dose-dependent over time. To overcome such limitations, and thereby ensure the continuous effective control of insect pests, we herein assessed the efficacy of binary mixtures of biological agents, with the aim of establishing an optimal ratio. The optimized mixture showed a significantly higher insecticidal effect, whereby biological pest control was considerably enhanced.•We combined Beauveria bassiana spores with azadirachtin, a chemical pesticide of botanical origin, and by assessing the efficacy of these two elements combined in different ratios, obtained an optimal formula. Additionally, we evaluated the compatibility between the two elements, and then assessed potential synergistic effects, as determined by the co-toxicity coefficient.•This protocol is dependent on the results of bioassays. It is not only suitable for combining fungal and chemical agents for the purposes of biocontrol but is also applicable to a variety of biological pesticides.•The evaluated binary mixture showed enhanced effective insect control. This approach will contribute to reducing applied dosages, thereby promoting efficient pest control and reducing application costs.
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