Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates
2015
Xia, Wei | Chen, Wei | Peng, Wei-fu | Li, Kun-tai
The aerobic Pseudomonas denitrificans is widely used for industrial and commercial vitamin B₁₂fermentation, due to its higher productivity compared to the anaerobic vitamin B₁₂-producing microorganisms. This paper aimed to develop a cost-effective fermentation medium for industrial vitamin B₁₂production by P. denitrificans in 120,000-l fermenter. It was found that maltose syrup (a low-cost syrup from corn starch by means of enzymatic or acid hydrolysis) and corn steep liquor (CSL, a by-product of starch industry) were greatly applicable to vitamin B₁₂production by P. denitrificans. Under the optimal fermentation medium performed by response surface methodology, 198.27 ± 4.60 mg/l of vitamin B₁₂yield was obtained in 120,000-l fermenter, which was close to the fermentation with the refined sucrose (198.80 mg/l) and was obviously higher than that obtained under beet molasses utilization (181.75 mg/l). Therefore, maltose syrups and CSL were the efficient and economical substrates for industrial vitamin B₁₂fermentation by P. denitrificans.
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