Quantification of histomorphometric and structural bone changes in a sheep model for fracture treatment in osteoporotic bone
2003
Lill, C.A. | Winterstein, E. | Eckhardt, C. | Rahn, B. | Goldhahn, J. | Schneider, E.
The histomorphometrical cancellous bone parameters of osteoporotic ewes were examined to establish a large animal model for the investigation of fracture treatment and healing in osteoporotic bone. Eighteen animals were divided into 2 groups. Group 1 (n = 8, age 3 to 5 years) served as an untreated control. In Group 2 (n = 10, age 7 to 9 years) osteoporosis was induced by means of ovariectomy, methylprednisolone application (average dose 160 mg/week) and Ca./Vit.D restricted diet during 7 months. Biopsies from iliac crest, femoral head and lumbar spine were harvested. Structural parameters from the biopsies were determined using µCT. In order to investigate static and dynamic parameters of bone remodelling, undecalcified sections were digitized and evaluated by means of image analysing software. Morphologically, bone area and trabecular thickness were reduced in osteoporotic sheep. Bone perimeter (-20%), trabecular number (-16%) and separation (+39%) showed a significant change in the iliac crest. The structure model index indicated a shift from the plate- to the rod-model. At different skeletal sites of osteoporotic sheep bone resorption was significantly increased (250- 675%), and bone formation significantly decreased (77-89%). Osteoid thickness (O.Th) was reduced (28-62%). In osteoporotic sheep the mean length of single labels was significantly shortened (64-73%) and the mineralising surface showed a significant decrease (80-92%). The mineralisation of the osteoid was comparable to the control group. The histomor-phometrical changes in this sheep model of osteoporo-sis are comparable to the human situation of a combined post-menopausal and steroid-induced osteoporosis.
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