Mapping Gene(s) for Grain Protein in Tetraploid Wheat (Triticum turgidum L.) Using a Population of Recombinant Inbred Chromosome Lines
1997
Joppa, L. R. | Du, Changheng | Hart, Gary E. | Hareland, Gary A.
A ‘Langdon’ durum wheat line with a pair of 6B chromosomes from an accession of Triticum turgidum L. var. dicoccoides (Körn. in litt., in Schweinf.) Bowden [LDN(DIC-6B)] previously was shown to have a gene(s) for high grain protein content (GPC). The objectives of this study were to develop a mapping population segregating only for genes on chromosome 6B, map the gene(s) for high GPC, and identify closely linked markers for use in marker-assisted breeding for this trait. The 6B mapping population was grown in replicated field trials and evaluated for GPC. A restriction fragment length polymorphism (RFLP) map of this population was available. The quantitative trait locus (QTL) analyses provided strong evidence that a gene(s) for high GPC (named QGpc.ndsu-6Bb) is located near the centromere of 6B. The most likely location for the gene(s) is in the short arm between Xabg387-6B and Xmwg79-6B. The logarithm of the odds (LOD) score for this interval is 18.9. Segregation in this segment accounted for 66% of the variation in GPC. Eleven additional markers have been mapped within 7 centimorgans (cM) of the midpoint of Xabg387-6B and Xmwg79-6B. One or more of these markers should be useful in marker-assisted breeding for high GPC in dorum wheat.
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