Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit (Citrus grandis) Segment Membrane
2014
Xiang, Yu | Cao, Jinping | Luo, Fenglei | Wang, Dengliang | Chen, Wei | Li, Xian | Sun, Chongde | Chen, Kunsong
A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ‐816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi‐preparative HPLC. Naringin was partially purified as a by‐product of this process using Sephadex LH‐20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC‐MS/MS.
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