Detection of Allergen Walnut Component in Food by an Improved Real-Time PCR Method
2009
Wang, Haiyan | Yuan, Fei | Wu, Yajun | Yang, Hairong | Xu, Baoliang | Zhongxue. | Chen, Ying
A real-time PCR method aimed at the gene sequence of the walnut vicilin-like seed storage protein was established for the detection of the allergen walnut in food. The primers and probe were designed based on published methods. The method provided positive results for walnut and negative results for other tested agricultural plant materials including pecan. The intrinsic detection limit of the method was 0.00125 ng of walnut DNA, and the practical detection limit was 0.001% (wt/wt) walnut content in wheat; both of these values are lower than that of previously published methods. Therefore, this real-time PCR method is sufficiently specific and sensitive for the detection of walnut component in food.
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