Identification and age-dependance of pteridines in the head of adult Mexican fruit fly, Anastrepha ludens
1996
Tomic-Carruthers, N. | Robacker, D.C. | Mangan, R.L.
Pteridines from head capsules of adult Anastrepha ludens were identified and evaluated as a tool for age determination of flies. Pteridines were identified by a combination of TLC, HPLC and UV and fluorescence spectroscopy. Tentative identifications were obtained for one red pterine, neodrosopterin, and three orange pterines, drosopterin, isodrosopterin and aurodrosopterin. Five pterines with blue or blue-green fluorescence were found and four were identified: 7,8-dihydrobiopterin; biopterin; pterin; and pterin-6-carboxylic acid. An additional blue fluorescing non-pteridine compound was identified as kyurenine. Two pterines with yellow fluorescence were identified as sepiapterin and deoxysepiapterin. Titers of sepiapterin and deoxysepiapterin were age dependent based on TLC and HPLC analyses. HPLC demonstrated that only deoxysepiapterin accumulated sufficiently and over a sufficiently long period of adult life to be useful as a quantitative tool for determining age of adult flies. The HPLC method developed in this work specifically for separation of deoxysepiapterin from sepiapterin and other pteridines used reverse-phase (C18) with a mobile phase of 30% methanol in water and detection at 420 nm.
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