Use of low cost agar alternative for in vitro propagation of commercially viable orchids is an attractive way for commercialization
2022
Deb, Chitta Ranjan | Pongener, Aolemla
Agar has been the popular choice of the masses as tissue culture gelling matrix. But, in culture medium one of the costlier components is agar which accounts for ∼38% of the media cost and puts a limitation for commercial exploitation of the technique. Different low cost substrata such as polyurethane foam, betel nut coir, coconut husk, paddy straw, forest leaf litter, sugarcane bagasse, moist cotton pad, saw dust etc. have been screened as they are cost effective and most of them are eco-friendly which could be used as complete agar alternative in orchid in vitro propagation. This communication reviews the possibilities of using different low cost substrata as agar alternative in orchid tissue culture to make the planting materials production platform more attractive for horticulturists. Further, these substrata can also be used with suitable modification for in vitro culture of non-orchid species which will help conserve the species economically.
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