Analysis of fumonisins in corn-based food by liquid chromatography with fluorescence and mass spectrometry detectors
2009
Silva, Liliana | Fernández-Franzón, Mónica | Font, Guillermina | Pena, Angelina | Silveira, Irene | Lino, Celeste | Mañes, Jordi
The presented procedure involves an extraction with methanol-water, centrifugation and cleanup with immunoaffinity columns. A comparison study between fluorescence detector, mass spectrometry, and tandem mass spectrometry with a triple quadrupole (QqQ) analyzer using an electrospray ionisation interface for the determination of fumonisin B₁ and B₂ in corn-based products has been performed. Limits of quantification obtained by the three detectors were lower than the maximum levels established by European Commission. Liquid chromatography coupled to tandem mass spectrometry provides higher sensitivity (12μgkg⁻¹for fumonisins B₁ and B₂) when compared to mass spectrometry (40μgkg⁻¹for both fumonisins), and fluorescence detection (20μgkg⁻¹ for fumonisin B₁ and 15μgkg⁻¹ for B₂), and also showed to be more precise. At 150 and 250μgkg⁻¹ spiking levels, the recovery rates for fumonisin B₁ and B₂ in corn products varied from 79% to 102%, with a relative standard deviation ranging from 9% to 17%. A critical assessment including advantages and drawbacks of each technique is presented. A total of 41 organic and non-organic corn-based food samples from Valencia markets were analyzed. Seven samples were contaminated with levels ranging from 68μgkg⁻¹ to 922μgkg⁻¹of fumonisin B₁ and 42μgkg⁻¹ to 640μgkg⁻¹of fumonisin B₂. Only one sample exceeded the maximum level for the sum of fumonisin B₁ and B₂, proposed for corn products in a recent EU regulation. The contamination frequency of organic corn samples (40%) was higher than non-organic ones (3.7%), and contained higher levels of fumonisin B₁ and B₂.
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