Sequence analysis of the cloned cucumis melo highly repetitive satellite dna
2014
Brennicke, A. | Hemleben, V.
The primary sequence of the prom inent Cucumis melo satellite DNA is presented. The cloned Hind III-repeat unit has a length of 352 bp and a GC-content of 56%. Within this sequence an inverted repeat of 9 bp, including a 286 bp loop, and one direct repeat are shown. Several open reading frames are possible following the sequence in both directions whose significance is not yet clear. The sequence has been compared with other highly repetitive DNA sequences of Sinapis alba and Scilla sibirica. The genomic satellite DNA exhibits an unusual behaviour in actinomycin D-CsCl gradients in comparison to ribosomal D NA
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